DMEM, Powder, High Glucose, Pyruvate12800017_济南隆炫科技发展有限公司

DMEM, Powder, High Glucose, Pyruvate12800017

更新时间:2021-03-26

点击数:442

描述 DMEM (Dulbecco's Modified Eagle Medium)是一种广泛使用的基础培养基,可用于支持很多种类的哺乳动物细胞生长。已经在 DMEM 培养基中培养成功的细胞包括原代成纤维细胞、神经元、胶质细胞、HUVEC 以及平滑肌细胞等原代细胞,还包括 HeLa、293、Cos-7和 PC-12等细胞系。针对广泛的细胞培养应用,提供了多种组分的 DMEM 改良培养基。使用培养基选择工具查找适合的配方。


这种 DMEM 的改良方式如下:

包含不含

•高糖•HEPES:

•L-谷氨酰胺•碳酸氢钠

•酚红

• 丙酮酸钠

DMEM 的使用

DMEM 与其他培养基的不同之处在于其含有相当于初始 Eagle's Minimal Essential Medium 4 倍浓度的氨基酸和维生素。DMEM 起初采用低糖 (1 g/L) 和丙酮酸钠配制,但常常在含/不含丙酮酸钠的高糖环境下使用。DMEM 不含蛋白质、脂质或生长因子。因此,DMEM 需要补充营养成分,通常需要添加 10% 胎牛血清 (FBS)。DMEM 培养基使用碳酸氢钠缓冲体系 (3.7 g/L),因此需要 5–10% CO2 的环境来维持生理 pH 值。粉末形式的 Gibco® 细胞培养基在配制时需要补充碳酸氢钠、调节 pH 并过滤(详情请参阅实验方案)。

品牌货号产品名称规格
GIBCO10010023PBS, pH 7.4500ml
GIBCO10010049PBS, pH 7.510*500ml
GIBCO11875093RPMI 1640 Medium500ml
GIBCO11875119RPMI 1640 Medium10*500ml
GIBCO11965092DMEM, high glucose500ml
GIBCO11965118DMEM, high glucose10*500ml
GIBCO11995073DMEM, high glucose, pyruvate10*500ml
GIBCO11995065DMEM, high glucose, pyruvate500ml
GIBCO11330032DMEM/F-12, HEPES500ml
GIBCO11330057DMEM/F-12, HEPES10*500ml
GIBCO11140050MEM Non-Essential Amino Acids Solution (100X)100ml
GIBCO11140076MEM Non-Essential Amino Acids Solution (100X)20*100ml
GIBCO10569044DMEM, High Glucose, GlutaMAX™, Pyruvate10*500ml
GIBCO10569010DMEM, High Glucose, GlutaMAX™, Pyruvate500ml
GIBCO10566024DMEM, High Glucose, GlutaMAX™ 10*500ml
GIBCO10566016DMEM, High Glucose, GlutaMAX™ 500ml
GIBCO22400105RPMI 1640 Medium, HEPES10*500ml
GIBCO22400089RPMI 1640 Medium, HEPES500ml
GIBCO15140163Penicillin-Streptomycin (10,000 U/mL)20*100ml
GIBCO15140122Penicillin-Streptomycin (10,000 U/mL)100ml
GIBCO25200056Trypsin-EDTA (0.25%), phenol red100ml
GIBCO25200072Trypsin-EDTA (0.25%), phenol red500ml
GIBCO25200114Trypsin-EDTA (0.25%), phenol red20*100ml
GIBCO17504044B-27 Supplement (50X), serum free10ml
GIBCO17502048N-2 Supplement (100X)5ml
GIBCOA1049101RPMI 1640 Medium (ATCC Modification)500ml
GIBCO35050061GlutaMAX Supplement100ml
GIBCO35050079GlutaMAX™ Supplement20 x 100 mL
GIBCOA3021002CTS™ AIM V™ SFM (RUO)1000ml
GIBCO12440061IMDM10*500ml
GIBCO12440053IMDM500ml
GIBCO31985070Opti-MEM I Reduced Serum Medium500ml
GIBCO31985088Opti-MEM I Reduced Serum Medium10*500ml
GIBCO21127022Ham's F-12K (Kaighn's) Medium500ml
GIBCO21127030Ham's F-13K (Kaighn's) Medium10*500ml
GIBCO14190144DPBS, no calcium, no magnesium500ml
GIBCO14190250DPBS, no calcium, no magnesium10*500ml
GIBCO11095080MEM500ml
GIBCO11095080MEM10*500ml
GIBCO25030164L-Glutamine (200 mM)20 x 100 mL
GIBCO25030081L-Glutamine (200 mM)100 mL
GIBCO10687010Hygromycin B (50 mg/mL)20 mL
GIBCOC10228Countess™ Cell Counting Chamber Slides 50 slides
GIBCOC10315Countess™ Cell Counting Chamber Slides 500slides
GIBCO12338018FreeStyle™ 293 Expression Medium1,000 mL
GIBCO12338026FreeStyle™ 293 Expression Medium6*1,000 mL
GIBCO10902088Sf-900™II SFM1,000 mL
GIBCO10902104Sf-901™II SFM6*1,000 mL
GIBCO41400045Insulin-Transferrin-Selenium (ITS -G) (100X)10 mL
GIBCO51500056Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)10 mL
GIBCO51300044Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X)10 mL
GIBCO18080044SuperScript™ III Reverse Transcriptase10000U
GIBCO18080051SuperScript™ III First-Strand Synthesis System50T
GIBCO13778150Lipofectamine™ RNAiMAX Transfection Reagent 1.5ml
GIBCO15250061Trypan Blue Solution, 0.4%100ml
GIBCOR25001Zeocin™ Selection Reagent8*1.25ml
GIBCO10687010Hygromycin B (50 mg/mL)20ml
GIBCO31800022RPMI 1640 Medium, powder10*1L
GIBCO12800017DMEM, powder, high glucose, pyruvate10*1L
GIBCO12100046DMEM, powder, high glucose10*1L
GIBCOA1113803Puromycin Dihydrochloride10*1ml
GIBCOA1113903Blasticidin S HCl (10 mg/mL)10*1ml
GIBCOQ32854Qubit™ dsDNA HS Assay Kit500 assays
GIBCOQ32856Qubit™ Assay Tubes 500 tubes
GIBCOA14524ExpiFectamine™ 293 Transfection Kit 
For 1 L of culture
GIBCOA1435101 
Expi293™ Expression Medium
1,000 mL
GIBCOA1435102Expi293™ Expression Medium6*1,000mL
GIBCOA29129ExpiFectamine™ CHO Transfection Kit 
For 1 L of culture
GIBCOA2910001ExpiCHO™ Expression Medium1000 mL
GIBCOA2910002ExpiCHO™ Expression Medium6*1,000mL
GIBCO10010023PBS, pH 7.4500ml
GIBCO10010049PBS, pH 7.510*500ml
GIBCO11875093RPMI 1640 Medium500ml
GIBCO11875119RPMI 1640 Medium10*500ml
GIBCO11965092DMEM, high glucose500ml
GIBCO11965118DMEM, high glucose10*500ml
GIBCO11995073DMEM, high glucose, pyruvate10*500ml
GIBCO11995065DMEM, high glucose, pyruvate500ml
GIBCOQ32854Qubit™ dsDNA HS Assay Kit500 assays
GIBCOQ32856Qubit™ Assay Tubes 500 tubes
GIBCO31800022RPMI 1640 Medium, powder10*1L
GIBCO12800017DMEM, powder, high glucose, pyruvate10*1L
GIBCO12100046DMEM, powder, high glucose10*1L
GIBCO12338018FreeStyle™ 293 Expression Medium1,000 mL
GIBCO12338026FreeStyle™ 293 Expression Medium6*1,000 mL
GIBCO17100017Collagenase, Type I, powder1g
GIBCO17101015Collagenase, Type II, powder1g
GIBCO17104019Collagenase, Type IV, powder1g
GIBCOA1148301CD FortiCHO™ 1000ml
GIBCO10743029CD CHO AGT™1000ml
GIBCOA2661501Dynamis™1000ml
GIBCO12563029TrypLE™ Select 500ML/瓶
GIBCO12309050OptiPRO™ SFM100ML/瓶
GIBCO0010057DG抗结团剂100ml/瓶
GIBCO0010057AE抗结团剂20ml/瓶
GIBCO10099141C胎牛血清500ml
GIBCO16010142新生牛血清1000ml
GIBCO0025062EJDMEM100L/桶干粉
GIBCO0835048SADMEM100L/桶干粉
GIBCO0035008EJM199100L/桶干粉
GIBCO21051040谷氨酰胺100L/桶干粉
GIBCO27250018胰酶100g干粉
GIBCO12500096DMEM/F-1250L/桶干粉
GIBCOA2503104EfficientFeed™ C+ AGT™1L/桶干粉
GIBCOA2503105EfficientFeed™ C+ AGT™10L/桶干粉
GIBCO12490025CD CHO AGT™10L/桶干粉
GIBCO0895086IN水解乳蛋白50L/桶干粉
GIBCO11800042水解乳蛋白5KG/桶干粉
GIBCO40203D磁珠10ml/瓶


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